Product Info Summary
SKU: | A06073 |
---|---|
Size: | 100μl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-V-ATPase B1 ATP6V1B1 Antibody
SKU/Catalog Number
A06073
Size
100μl
Form
Liquid
Description
Boster Bio Anti-V-ATPase B1 ATP6V1B1 Antibody catalog # A06073. Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-V-ATPase B1 ATP6V1B1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06073)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from the C-terminal region of human V-ATPase B1. at AA rangle: 350-430
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06073 is reactive to ATP6V1B1 in Human, Mouse
Applications
A06073 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
56.833kDa
Background of ATP6V1B1
Cyclin T2 is a ubiquitously expressed nuclear protein that is a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns that contribute to the temporal coordination of each mitotic event. This cyclin and its kinase partner CDK9 were found to be subunits of the transcription elongation factor p-TEFb. The p-TEFb complex containing this cyclin was reported to interact with, and act as a negative regulator of human immunodeficiency virus type 1 (HIV-1) Tat protein. Anti-Cyclin T2 Antibody is useful for researchers interested in HIV research, Gene expression, and cell cycle pathways.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of specific cells using V-ATPase B1 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For ATP6V1B1 (Source: Uniprot.org, NCBI)
Gene Name
ATP6V1B1
Full Name
V-type proton ATPase subunit B, kidney isoform
Weight
56.833kDa
Superfamily
ATPase alpha/beta chains family
Alternative Names
ATP6B158kD subunit; ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1; Endomembrane proton pump 58 kDa subunit; H+-ATPase beta 1 subunit; vacuolar proton pump 3; Vacuolar proton pump subunit B 1; vacuolar proton pump, subunit 3; VATBMGC32642; V-ATPase B1 subunit; V-ATPase subunit B 1; Vma2; V-type proton ATPase subunit B, kidney isoform ATP6V1B1 ATP6B1, DRTA2, RTA1B, VATB, VMA2, VPP3 ATPase H+ transporting V1 subunit B1 V-type proton ATPase subunit B, kidney isoform|ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1|H(+)-transporting two-sector ATPase, 58kD subunit|H+-ATPase beta 1 subunit|V-ATPase B1 subunit|V-ATPase subunit B 1|endomembrane proton pump 58 kDa subunit|vacuolar proton pump 3|vacuolar proton pump subunit B 1|vacuolar proton pump, subunit 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATP6V1B1, check out the ATP6V1B1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATP6V1B1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-V-ATPase B1 ATP6V1B1 Antibody (A06073)
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No publications found for A06073
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-V-ATPase B1 ATP6V1B1 Antibody
Question
I would like to test anti-V-ATPase B1 antibody A06073 on human adult mammalian kidney for research purposes, then I may be interested in using anti-V-ATPase B1 antibody A06073 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
The products we sell, including anti-V-ATPase B1 antibody A06073, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-24
Question
Is a blocking peptide available for product anti-V-ATPase B1 antibody (A06073)?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
We do provide the blocking peptide for product anti-V-ATPase B1 antibody (A06073). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-13
Question
We are currently using anti-V-ATPase B1 antibody A06073 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on pig tissues as well?
S. Anderson
Verified customer
Asked: 2019-11-08
Answer
The anti-V-ATPase B1 antibody (A06073) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-08
Question
See attached the WB image, lot number and protocol we used for adult mammalian kidney using anti-V-ATPase B1 antibody A06073. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-31
Question
I see that the anti-V-ATPase B1 antibody A06073 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-01-01
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-01-01
Question
Is this A06073 anti-V-ATPase B1 antibody reactive to the isotypes of ATP6V1B1?
R. Williams
Verified customer
Asked: 2017-09-26
Answer
The immunogen of A06073 anti-V-ATPase B1 antibody is Synthesized peptide derived from the C-terminal region of human V-ATPase B1. at AA rangle: 350-430. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-09-26
Question
I was wanting to use your anti-V-ATPase B1 antibody for WB for human adult mammalian kidney on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human adult mammalian kidney identification?
Verified Customer
Verified customer
Asked: 2017-09-07
Answer
As indicated on the product datasheet, A06073 anti-V-ATPase B1 antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human adult mammalian kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-09-07